A reaction mixture consisting primers, DNA polymerase and chromosomal DNA was centrifuged and placed in a thermal cycler to perform 20 cycles of PCR that undergoes denaturation, annealing and primer extension during each cycle. After which, the PCR products will be detected by agarose gel electrophoresis followed by visualization under a UV transilluminator to match with marker DNA as so to identify the foodborne pathogens.
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