Principles of genetic modifying processes of CORN

Among the 6 discussed GM processes used in the previous post, 3 of them are used to produce GM corn.

1.) Electroporation
2.) Biolistics
3.) Bacteria Carriers

In addition, a less commonly used technique is used to modify GM corn.
4.) Whiskers-Mediated Transformation
This method relies on the use of silicon carbide fibres. Selected target cells, fibers and plasmid DNA are placed in a tube and mixed by vortex treatment in a prescribed manner. The main mechanism lies with the silicon carbide fibers function as needles enable the entry of DNA into cells during the mixing process. The treated corn tissue is then plated on a selection media of the regeneration of GM seedlings.

Explanatory diagram on whishers mediated transformation:

Materials needed for the modifying process:

After adding into the tube, the tube undergoes vortex treatment to allow DNA to gain entry to the target cell.